Researcher (Work from Home)

Chapter 4: Jul 2024 - Jun 2025 ( 1 year ) NUGEN INDUSTRIES, located in U.S.A Position: Resear cher, Work from Home

• Key responsibilities − Resear ch the used lab equipment, parts, and consumables (including dismantled units) designed for biological, medical, and microbiological laboratories. Write clear, detailed descriptions and specifications. Then list the items on eBay, including the name, model, part number, serial number, expiration date, manufacturer, price, full description, technical specifications, and high-quality photos and videos − Update eBay IDs and item locations in the Inventory Master File − Cr eate labels and Stock Keeping Units (SKUs) for the items − Resear ch market prices for the items

Chapter 2: Oct 2014 - Dec 2021 ( 7 years 3 months ) Oxford University Clinical Resear ch Unit, located in Vietnam ( OUCRU ) ▪ Position: Resear ch Assistant ▪ Key responsibilities  Spearheaded the development of conjugate vaccine for Typhoid through the execution of in vitro experiments, including the following:

• Macrophage cell culture: Conduct Cell Death Assay infected with various pathogens such as S. Typhi, S. Paratyphi A, S. Typhimurium and E. Coli
• Protein expression and purification: The sequence of those S. Typhi candidate antigens cloned into 5’NcoI and 3’NotI restriction sites of pET28b for His-Tag purification with E. Coli BL21(DE3) pLysS for expression and purification soluble and insoluble recombinant protein
• Enzyme-Linked ImmunoSorbent Assay: ELISAs employed to detect antigens specific antibodies class IgA, IgM and IgG with acute, convalescent, asymptomatic chronic and longitudinal immune response in ALS and human plasma typhoid fever that confirmed S. Typhi (ST), S. Paratyphi A (SPA) or other infections, undiagnosed febrile disease and negative control population
• Immuno-strip for rapid diagnostic test: The candidate S. Typhi antigens were immobilized on a nitrocellulose membrane and subsequently tested with ST, SPA, and undiagnosed febrile plasma samples
• Immuno-fluorescence staining assay: The expression of S. Typhi antigens was visualized, with images captured using a wide-field fluorescent microscope
• Serum Bactericidal Assay (SBA): Mice sera, immunized to different specific antigens or clinical typhoid patient sera to kill wild-type or mutant S. Typhi and S. Paratyphi A strains
• Knockout genes of interested: Homologous recombination with site-specific mutagenesis
• Transposon Directed Insertion Sequencing-TraDIS: The mutant library with clinical S. Typhi and S. Paratyphi A isolates were generated by Tn5 transposon
• Molecular biology: Techniques employed included DNA, RNA, Protein extraction, PCR, gene sequencing, flow cytometry, electroporation transformation, and SDS PAGE Microbiology: Microbiological cultures, and testing methodologies pertinent to research and diagnostic laboratories, as well as pharmaceutical manufacturing, were conducted  R and Python programming software: statistic, analysis, visualization, plot with meta data of Serology, SBA, TraDIS, Genome, OMIC and Cytokines, S. Typhi genes functional classification using KEGG pathways or Gen Ontology  Clinical Research Associate: collect, manage and analysis Case Report Form (CRF)  Typhoid Clinical Vaccine Trial: TyVAC study and STRATAA study