Group Leader

Research Interests

• Recombinant Therapeutic Proteins Production (Via Genetic Engineering)
• Treatment By Stem Cells (Via Animal Models)
• Molecular Biology (Via Pcr And Real-time Pcr)
• Synthetics Biology (Via Bioinformatics)

Skills In The Research Lab

• Basic Bioinformatics Softwares
• Genomic Dna And Plasmid Extraction
• Manipulation With Assembly And Amplification Pcr
• Ability To Deal With Troubleshooting Of Pcr
• Ability To Deal With Troubleshooting Of Real-time Pcr
• Gene Cloning And Expression
• Restriction Enzymes Manipulation
• Gene Reorientation To Be In An Open Reading Frame
• Rna Extraction
• Cdna Reaction
• Protein Expression Optimization
• Elisa Assay
• Western Blot Assay
• Tissue Staining H&e
• Protein Electrophoresis
• Protein Purification And Refolding
• Mammalian Culture Manipulation And Preservation
• Anticancer Activity Evaluation Of Recombinant Therapeutic Proteins In Tissue Culture Systems
• Antiviral Activity Evaluation Of Recombinant Therapeutic Proteins In Tissue Culture Systems
• Mtt Assay For Assessing Cell Viability
• Mesenchymal Stem Cell Manipulation And Preservation
• Rat Manipulation (Oral, Ip, And Iv Injection)
• Rat Dissection And Blood Collection
• Manipulation And Growth Optimization Of Recombinant E. Coli
• Manipulation And Set Up The Ideal Conditions For Fungi Production For Biopolymer And Lipids
• Molecular Identification Of Different Fungi Using Genetic Methods (18s, 28s)
• Basic Manipulation With Medical Bacteria
• Microscopic Examination For Malaria
• Calculations In Analytical Chemistry
• Calculations In Molecular Biology

Research Projects

• Production Of Two Active Recombinant Human Interferons
• Production Of Lipids And Biopolymers From Fungi

Instruments

• Thermal Cycler (Pcr) System
• Real-time Pcr
• Elisa Micro Plate Reader
• Spectrophotometer
• Fplc (Fast Performance Liquid Chromatography)
• Sonicator
• Gel Documentation System
• Dna And Protein Electrophoresis Units
• Training On 2d Protein Electrophoresis System
• Training On Confocal Microscope
• Training On Flow Cytometer
• Nano Drop Instrument