Stacy M. - Assistant Professor

New Haven, United States

Experience

Jan 2021 - Present

4 years 11 months

New Haven, United States

Developing mass spectrometry technologies to unravel the complex role of mucin-domain glycoproteins in disease
Advising postdoctoral fellows, graduate students, and undergraduate students in scientific exploration, manuscript preparation, and science communication
Teaching undergraduate and graduate level analytical chemistry, chemical biology, and quantitative instrumentation

Mar 2018 - May 2018

3 months

Lille, France

Developed protocols for on-tissue glycan and glycopeptide imaging of canine glioma tissues by MALDI imaging mass spectrometry (IMS)
Operated three MALDI-IMS instruments, including: Bruker UltraFlex, Bruker RapiFlex, and Thermo MALDI-Orbitrap
Investigated macrophage reactivation through proprotein convertase 1/3 inhibition

Jan 2016 - Dec 2020

5 years

Stanford, United States

Characterized metalloproteases that cleave mucin proteins to assist in proteomic classification and enrichment of mucin-like glycoproteins
Operated, troubleshooted, and maintained a Thermo Orbitrap Fusion Tribrid high-resolution mass spectrometer
Collaborated in several mass spectrometry-related projects, including bump-and-hole engineering for O-glycoproteomics, characterization of AAV viral capsid post-translational modifications, and site-localization of Siglec-7 glycan binding partners

Apr 2014 - May 2014

2 months

Birmingham, United Kingdom

Isolated human leukocyte antigen (HLA)-associated peptides from normal and tumor lung tissue samples
Performed 7-day interferon-γ enzyme-linked immunospot (ELISpot) assays to measure immunogenicity of peptide antigens
Executed intracellular cytokine staining (ICS) to assess memory T-cell responses

Jan 2014 - Dec 2016

3 years

Charlottesville, United States

Developed phenylboronic acid solid phase extraction enrichment procedure to selectively isolate, enrich, and identify glycosylated MHC class I and II associated peptides
Operated and maintained three low-resolution (LTQ) and four high-resolution mass spectrometers (Thermo Fusion Tribrid, LTQ-Velos Elite, LTQ-Orbitrap, LTQ-FTICR)
Analyzed CAD, HCD, and ETD MS2 spectra by de novo sequencing, manual spectra interpretation, and validation of computer-based search algorithm results (Mascot, OMSSA, Byonic)

Jan 2010 - Dec 2014

5 years

Charlottesville, United States

Developed phenylboronic acid solid phase extraction enrichment procedure to selectively isolate, enrich, and identify glycosylated MHC class I and II associated peptides
Operated and maintained three low-resolution (LTQ) and four high-resolution mass spectrometers (Thermo Fusion Tribrid, LTQ-Velos Elite, LTQ-Orbitrap, LTQ-FTICR)
Analyzed CAD, HCD, and ETD MS2 spectra by de novo sequencing, manual spectra interpretation, and validation of computer-based search algorithm results (Mascot, OMSSA, Byonic)

Jan 2005 - Dec 2010

6 years

Ann Arbor, United States

Manufactured custom peptides including synthesis, purification, and quality control
Operated multiple peptide synthesizers in addition to analytical and preparatory scale HPLCs
Analyzed peptide samples via ESI-MS on an LCQ and MALDI-TOF for quality control
Prepared and analyzed client protein samples for N-terminal sequencing via Edman degradation