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Dennis Dannecker

Cryo-STEM of Biomacromolecules

Dennis Dannecker
Cologne, Germany

Experience

Cryo-STEM of Biomacromolecules

Forschungszentrum Jülich (ERC-3)

  • Developed and applied Cryo Scanning Transmission Electron Microscopy (STEM) imaging techniques (ptychography, iDPC) for 3D structural determination of biological samples
  • Utilized cryo-specimen preparation methods and integrated computational workflows

Radiolabeled sdAb Characterization

Helmholtz Zentrum Dresden Rossendorf

  • Expression and purification of single-domain antibodies (Nanobodies)
  • Site-specific bioconjugation of Nanobodies with radionuclide chelators for efficient radio-labeling
  • Performed in vitro assays for validation and optimization of conjugation and labeling

Antibiotic Resistance in Bacillus subtilis

Michigan State University

  • Investigated the BceAB module and its interaction with bacitracin
  • Conducted molecular cloning, protein expression, membrane protein purification, nanodisc assembly, microscale thermophoresis and spectral shift binding assays, growth and signaling assays

Characterization of Cryptochromes

University of Cologne

  • Expression and purification of light sensitive cryptochromes
  • Biochemical characterization and analysis using UV/Vis kinetics, SEC, blue native PAGE and negative-stain electron microscopy

Summary

Master's student in Biochemistry with practical experience in structural biology, electron microscopy and protein-ligand interactions. Interested and passionate about methods and technology in life science and their development. Seeking to contribute to innovative projects at the interface of biology, chemistry, physics and bioengineering.

Skills

Experimental:

  • Protein Purification (Fplc And Hplc, Gel Filtration And Affinity Chromatography)
  • Membrane Protein Work
  • Radiolabeling
  • Cryo-em Sample Preparation
  • Negative Staining For Electron Microscopy
  • Uv/vis Spectroscopy
  • Microscale Thermophoresis
  • Site-directed Mutagenesis
  • Cell Culture
  • Sirna Knockdown
  • Western Blotting
  • Fluorescence Microscopy

Computational & Software:

  • Cryosparc
  • Relion
  • Cryolo
  • Chimera
  • Chimerax
  • Pymol
  • Origin
  • Benchling
  • Clc Workbench
  • Image Analysis (Lasx, Image Lab)
  • Data Documentation (Elab)
  • Excel
  • Prism
  • Python Scripting

Soft Skills:

  • Collaborative And Method-driven Research Approach

Languages

German
Advanced
English
Advanced

Education

Oct 2022 - Present

University of Cologne

M.Sc. Biochemistry · Biochemistry · Cologne, Germany · 1.1 (A)

Oct 2017 - Sep 2022

Ulm University

B.Sc. Biochemistry · Biochemistry · Ulm, Germany · 1.2 (A)

Profile

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Frequently asked questions

Do you have questions? Here you can find further information.

Where is Dennis based?

Dennis is based in Cologne, Germany.

What languages does Dennis speak?

Dennis speaks the following languages: German (Advanced), English (Advanced).

What roles would Dennis be best suited for?

Based on recent experience, Dennis would be well-suited for roles such as: Cryo-STEM of Biomacromolecules, Radiolabeled sdAb Characterization, Antibiotic Resistance in Bacillus subtilis.

What is Dennis's latest experience?

Dennis's most recent position is Cryo-STEM of Biomacromolecules at Forschungszentrum Jülich (ERC-3).

What is Dennis's education?

Dennis holds a Master in Biochemistry from University of Cologne and a Bachelor in Biochemistry from Ulm University.

What is the availability of Dennis?

Dennis will be available part-time from March 2026.

What is the rate of Dennis?

Dennis's rate depends on the specific project requirements. Please use the Meet button on the profile to schedule a meeting and discuss the details.

How to hire Dennis?

To hire Dennis, click the Meet button on the profile to request a meeting and discuss your project needs.

Average rates for similar positions

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Market avg: 630-790 €
The rates shown represent the typical market range for freelancers in this position based on recent contracts on our platform.
Actual rates may vary depending on seniority level, experience, skill specialization, project complexity, and engagement length.