Dennis Dannecker
Cryo-STEM of Biomacromolecules
Experience
Cryo-STEM of Biomacromolecules
Forschungszentrum Jülich (ERC-3)
- Developed and applied Cryo Scanning Transmission Electron Microscopy (STEM) imaging techniques (ptychography, iDPC) for 3D structural determination of biological samples
- Utilized cryo-specimen preparation methods and integrated computational workflows
Radiolabeled sdAb Characterization
Helmholtz Zentrum Dresden Rossendorf
- Expression and purification of single-domain antibodies (Nanobodies)
- Site-specific bioconjugation of Nanobodies with radionuclide chelators for efficient radio-labeling
- Performed in vitro assays for validation and optimization of conjugation and labeling
Antibiotic Resistance in Bacillus subtilis
Michigan State University
- Investigated the BceAB module and its interaction with bacitracin
- Conducted molecular cloning, protein expression, membrane protein purification, nanodisc assembly, microscale thermophoresis and spectral shift binding assays, growth and signaling assays
Characterization of Cryptochromes
University of Cologne
- Expression and purification of light sensitive cryptochromes
- Biochemical characterization and analysis using UV/Vis kinetics, SEC, blue native PAGE and negative-stain electron microscopy
Summary
Master's student in Biochemistry with practical experience in structural biology, electron microscopy and protein-ligand interactions. Interested and passionate about methods and technology in life science and their development. Seeking to contribute to innovative projects at the interface of biology, chemistry, physics and bioengineering.
Skills
Experimental:
- Protein Purification (Fplc And Hplc, Gel Filtration And Affinity Chromatography)
- Membrane Protein Work
- Radiolabeling
- Cryo-em Sample Preparation
- Negative Staining For Electron Microscopy
- Uv/vis Spectroscopy
- Microscale Thermophoresis
- Site-directed Mutagenesis
- Cell Culture
- Sirna Knockdown
- Western Blotting
- Fluorescence Microscopy
Computational & Software:
- Cryosparc
- Relion
- Cryolo
- Chimera
- Chimerax
- Pymol
- Origin
- Benchling
- Clc Workbench
- Image Analysis (Lasx, Image Lab)
- Data Documentation (Elab)
- Excel
- Prism
- Python Scripting
Soft Skills:
- Collaborative And Method-driven Research Approach
Languages
Education
University of Cologne
M.Sc. Biochemistry · Biochemistry · Cologne, Germany · 1.1 (A)
Ulm University
B.Sc. Biochemistry · Biochemistry · Ulm, Germany · 1.2 (A)
Profile
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