Led multiple independent projects investigating molecular mechanisms of transcriptional regulation in Vibrio cholerae using molecular biology and genetics
Executed ChIP-seq and Tn-seq experiments, including NGS library preparation for Illumina sequencing, quality control, and downstream analysis
Developed and optimized novel sequencing-based assays to analyze membrane-chromosome interactions in vivo, including protocol design, troubleshooting, documentation
Designed and performed high-throughput genetic screens using transposon mutagenesis and error-prone PCR-generated libraries to isolate mutants of interest
Generated mutants to test via western blot, qPCR & qRT-PCR, fluorescence microscopy, ChIP
Designed primers and used SOE PCR to build reporter constructs and protein fusions
Supervised multiple graduate and undergraduate researchers in molecular cloning, sequencing workflows, and data analysis, contributing to multiple publications
Collaborated with other scientists to conduct interdisciplinary research
Published two first-author manuscripts; presented work at national conferences, weekly lab meetings
Jan 2017 - Dec 2019
3 years
Irving, United States
Undergraduate Researcher, Department of Biology
University of Dallas
Studied the coevolution of virulence and resistance in Pseudomonas aeruginosa using Drosophila melanogaster oral infection models
Mentored junior lab members in experimental techniques and background