My (T.) C.
AI Expert Contributor
Experience
Jan 2025 - Present
11 monthsAI Expert Contributor
SnorkelAI
- Generated and peer reviewed cellular and molecular biology questions to train LLM
Oct 2022 - Mar 2024
1 year 6 monthsScientist, Cell Biology
Tyra Biosciences
- Successfully conducted compound screening using NanoBRET assay for achondroplasia, demonstrating proficiency in advanced biochemical assay techniques and drug discovery
- Conducted cell viability assay using the CellTiter-Glo across multiple cancer cell lines to evaluate therapeutic efficacy and support target validation studies
- Designed and executed signal transduction study to study downstream effects to guide clinical molecule optimization
- Measured phosphorylation and total protein with ELISA and AlphaLISA for proof-of-concept study
- Provided support to the in vivo team by providing cancer cells for inoculations ensuring continuity of critical in vivo studies
- Optimized siRNA knockdown in various cancer and neuroblastoma cell lines, enhancing target validation and advancing therapeutic research
- Mentored junior scientists on assay design and execution, accelerating team’s project timelines by training in assay setup and data interpretation
Jan 2022 - Sep 2022
9 monthsScientist I, Discovery Technology
Plexium
- Supported high-throughput screening and targeted protein degradation projects by designing assays and conducting signal transduction studies for drug discovery focusing on E3 ligase expression
- Screened cell line for expression of E3 ligases
- Established stable cell lines for screening
- Designed and executed knockdown with siRNA/shRNA in SH-SY5Y for antibody validation
- Presented findings in meetings to the team and external collaborators, facilitating data-driven discussions and strategic decision-making
- Managed external collaborations with CROs to accelerate project timelines, coordinate assay development, ensure alignment on project deliverables
- Managed part-time lab manager to ensure smooth lab operations
Apr 2021 - Jan 2022
10 monthsSenior Research Associate, Discovery Technology
Plexium
- Designed and executed signal transduction study to validate antibodies for screening
- Designed and executed siRNA knockdown and utilized known compounds to study signal transduction to validate antibodies across multiple cell lines
- Used ultra high throughput devices to validate antibodies
Jan 2021 - Apr 2021
4 monthsSenior Research Associate (Contractor) at Ferring Institute
BioPhase
- Participated in two different projects to support discovery towards preclinical programs
- Designed and executed signal transduction studies to examine the neutralizing effects of compound on inflammatory pathway using HTRF assay
Oct 2019 - Jul 2020
10 monthsScientist II
Comet Therapeutics
- Designed, developed, and performed dd-PCR studies for mechanism of action studies
- Examined effects of compounds on insulin-mediated glucose uptake in human myotubes
- Designed, developed, and evaluated the efficacy of compounds on fatty acid oxidation, mitochondrial function and glycolytic function in patient-derived fibroblasts using the Seahorse Analyzer
- Designed, developed, and performed hexokinase activity, citrate synthase, pH, pyruvate and lactate measurements for mechanism of action studies
- Managed and mentored a Research Associate, providing guidance on experimental design, data analysis, and professional development
- Worked as a team to assist in patent filing
Feb 2015 - Oct 2019
4 years 9 monthsScientist, Cardio-Renal Metabolic Ophthalmic Diseases
Merck Research Laboratories
- Collaborated on five preclinical projects to support discovery that leads to Go/No-Go decisions, lead optimization, and preclinical programs
- Isolated and cultured ADSC and satellite cells from mouse tissues
- Developed and validated second messengers, cAMP and cGMP measurements to support fibrosis studies using liver and lung cell models, contributing to preclinical program decision-making
- Conducted siRNA screen to identify key targets regulating mitochondrial function, utilizing Seahorse analysis, qRT-PCR and Western blotting to assess cellular bioenergetics and protein expression changes
- Maintained detailed experimental records, data logs, and reports to ensure reproducibility
- Assisted in setting up and optimizing the lab to ensure efficient research workflows
- Maintained laboratory equipment, supplies, and reagents to support seamless experimentation
- Managed internal collaborations with other departments within Merck and external collaborations with academic lab and CROs
Apr 2013 - Dec 2014
1 year 9 monthsSenior Research Associate, Mammalian Cell Culture
Ember Therapeutics
- Conducted siRNA screen to identify key targets regulating mitochondrial function, utilizing Seahorse analysis, immunofluorescence and microscopy to assess cellular bioenergetics and morphological changes
- Designed and executed biochemical and cellular assays for target identification and validations such as Seahorse analysis, qRT-PCR, ELISA, and WB
- Characterized brown/white adipocytes utilizing qRT-PCR, Seahorse analysis, and Mito-Tracker Green and Red to assess gene expression, metabolic activity, and mitochondrial dynamics
- Provided support and guidance to other team members
Jun 2001 - Apr 2013
11 years 11 monthsResearch Associate IV/Lab Manager
University of Massachusetts Medical School
- Designed and executed a screening project utilizing siRNA to study co-repressors and co-activators of transcription factors on mitochondrial biogenesis
- Studied the effect of in vivo knock down of macrophages
- Collaborated with a team of scientists to identify adipocyte progenitor cells and investigate the role of insulin and adipose angiogenesis in a diet-induced obese model
- Isolated and cultured primary mouse cells such as monocytes, ADSC and satellite cells
- Managed a mouse colony and diet-induced mouse model, genotyping, dissections, and general husbandry
- Maintained detailed experimental records, data logs, and reports to ensure reproducibility
- Maintained laboratory equipment, supplies, and reagents to support seamless experimentation
- Trained and collaborated with different groups such as interns, graduate students, post-docs and CROs in different lab techniques
Summary
Experienced and versatile scientist with over 20 years in biotechnology and drug discovery across cardio-metabolic, obesity, type II diabetes, rare disease, oncology, and neurodegeneration.
Expertise in molecular biology techniques, cell-based assays, and preclinical studies, with a focus on screening, target validation, siRNA, protein degradation, and signal transduction.
Recognized for proficiency in high-throughput screening, energy metabolism, mitochondria bioenergetics, and collaboration with cross-functional teams to advance pipeline progression.
Languages
English
AdvancedEducation
Oct 2003 - Jun 2005
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Master of Science · Biotechnology
Oct 2000 - Jun 2002
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Bachelor of Science · Biology
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Associate of Arts · Arts
Certifications & licenses
Patent Law
University Of Pennsylvania
Understanding Intellectual Property
LinkedIn Learning
Understanding Us Patents: A Deeper Dive
LinkedIn Learning
Clinical Trials Analysis, Monitoring, And Presentation
John Hopkins
Clinical Trials Data Management And Quality Assurance
John Hopkins University
Clinical Trials Management And Advanced Operations
John Hopkins University
Design And Conduct Of Clinical Trials
John Hopkins University
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